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"Epigenetic Modification Screening Trends 2014" market report summarizes the results of HTStec’s 3rd industry-wide global web-based benchmarking survey on epigenetic modification screening carried out in May 2014. The main questionnaire consisted of 29 multi-choice questions and 2 open-ended questions. In addition, there were 9 questions related solely to survey demographics. The survey collected 91 validated responses, of these 65% provided comprehensive input. Survey responses were geographically split: 65% North America; 25% Europe; 7% Asia (excluding Japan); 2% Japan; and 1% China. Survey respondents were drawn from persons or groups performing epigenetic modification screening assays or planning future investigation in this area.
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The survey looked at the following aspects of epigenetic modification screening, as practiced today (2014) and in some cases as predicted for the future (2016):
· Opinion on the current status of epigenetic targets
· Key diseases/therapeutic area(s) investigating epigenetic modification
· Level of priority for epigenetic modification research
· Epigenetic modification classes of greatest interest, and where respondents have investigated assay feasibility or already performed in house or outsourced testing
· Main source/origin of epigenetic proteins used
· Stages in the drug discovery process using biochemical assays and/or cellular assays for epigenetic modification
· Epigenetic modifications proteins with adequate biochemical and cellular assays
· Preferred biochemical and cellular assay format/detection technologies for internal work and when outsourcing testing against epigenetic modifications proteins;
· Substrates most commonly used for epigenetic assays
· The challenges of assay development of epigenetic modification proteins; new tools required to drive the investigation of epigenetic modification assays
· What limits epigenetic modification screening
· Aspects of epigenetic modification drug discovery that are most limiting today
· Where the situation concerning the main limitations of epigenetic modification screening assays has improved in the past 2 years
· Number of FTE devoted to epigenetic modification research and different targets supported
· Number of epigenetic modification primary screens and wells per screen
· Approach to the primary screening (HTS) of epigenetic targets
· Proportion of epigenetic primary screens that are biochemical assays
· In house epigenetic modification assay reagent budget and breakdown into components purchased
· Main suppliers of reagents and tools used to assay epigenetic modifications in house
· Average material cost per well of epigenetic modification screening assays
· Interest in outsourcing epigenetic modification research
· Proportion of epigenetic modification testing outsourced and number of wells outsourced
· Budget for outsourcing epigenetic modification assays and services
· Preferred fee-for-service providers for outsourced epigenetic modification profiling assays
· Unmet needs in epigenetic enzyme assays and screening today
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A median of 21-30% of all epigenetic primary screens were enzyme/biochemical assays in 2014. A median budget of $5K-$25K/lab was allocated for epigenetic enzyme assay reagents in 2014. A bottom-up model developed around respondent’s annual budget for epigenetic modification assay reagents estimated the global market to be around $52M in 2014. The greatest share of this market was allocated to assay specific probes and biochemical assay kits. The most used commercial sources of reagents and tools for in house epigenetic modification assays were Life Technologies, Abcam and Sigma Aldrich. The median cost per single well for epigenetic modification assays undertaken in house was $0.75-$1.0 for biochemical assays versus $1-$2 for cellular assays. Profiling against panels of epigenetic targets using biochemical or cellular assays were the aspects of epigenetic modification research most respondents were interested to outsource. The median % of epigenetic modification primary screening outsourced in 2014 was ‘none’. The median % of epigenetic modification profiling outsourced in 2014 was <10%. The median total number of single wells outsourced to a fee-for-service provider for epigenetic primary screening and profiling was <100 wells in 2014.
A bottom-up model developed around respondent’s annual budget for outsourced epigenetic modification testing and services estimated the global market to be around $4M in 2014. The preferred fee-for-service providers of epigenetic modification profiling assay services were Life Technologies, Cisbio and Eurofins. The full report provides the data, details of the breakdown of the responses for each question, its segmentation and the estimates for the future (2016). It also highlights some interesting differences, particularly between Pharma versus the other survey groups.
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